Cohomology of Cryo-Electron Microscopy

The goal of cryo-electron microscopy (EM) is to reconstruct the 3-dimensional structure of a molecule from a collection of its 2-dimensional projected images. In this paper, we show that the basic premise of cryo-EM—patching together 2-dimensional projections to reconstruct a 3-dimensional object—is naturally one of Čech cohomology with SO(2)-coefficients. We deduce that every cryoEM reconstruction problem corresponds to an oriented circle bundle on a simplicial complex, allowing us to classify cryo-EM problems via principal bundles. In practice, the 2-dimensional images are noisy and a main task in cryo-EM is to denoise them. We will see how the aforementioned insights can be used towards this end.